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GST anticorps (Alkaline Phosphatase (AP))

Cet anticorps anti-GST est un anticorps Lapin Polyclonal détectant GST dans WB, ELISA et IHC. Adapté pour Schistosoma japonicum.
Rockland
N° du produit ABIN964665
N° du produit (Fournisseur): 600-405-200

Aperçu rapide pour GST anticorps (Alkaline Phosphatase (AP)) (ABIN964665)

Antigène

Voir toutes GST Anticorps
GST (Glutathione S Transferase (GST))

Reactivité

  • 96
  • 29
  • 14
  • 9
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  • 3
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  • 1
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Schistosoma japonicum

Hôte

  • 64
  • 52
  • 49
  • 2
  • 1
  • 1
Lapin

Clonalité

  • 99
  • 68
Polyclonal

Conjugué

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  • 4
  • 3
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  • 1
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Cet anticorp GST est conjugé à/à la Alkaline Phosphatase (AP)

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • N° du produit (Fournisseur)

    600-405-200

    Fournisseur

    Rockland

    Fonction

    GST Antibody Alkaline Phosphatase Conjugated

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

    Attributs du produit

    Synonyms: rabbit anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

    Stérilité

    Sterile filtered

    Immunogène

    Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

    Immunogen Type: Native Protein

    Isotype

    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:200-1:1,000

    Application Note: Anti-Glutathione-S-Transferase Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

    Western Blot Dilution: 1:500-1:2,500

    ELISA Dilution: 1:2,000-1:10,000

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Stockage commentaire

    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

    Date de péremption

    12 months
  • Antigène

    GST (Glutathione S Transferase (GST))

    Autre désignation

    GST

    Sujet

    Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
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